A recent study has identified potential anti-malaria properties in the root of Cissampelos pareira, a plant traditionally used in India as an anti-snake venom. The study, conducted by Bhatt et al. and published in the Journal of Ethnopharmacology, focused on evaluating the anti-plasmodial potential of different crude extracts and fractions of the plant, as well as developing a method for the quantification and identification of active compounds.
Summary:
- Cissampelos pareira is traditionally used in India as an anti-snake venom, but its anti-malaria properties were previously unknown.
- The study by Bhatt et al. focused on evaluating the anti-plasmodial potential of different crude extracts and fractions of the plant, as well as developing a method for the quantification and identification of active compounds.
- Five compounds were quantified using UPLC-DAD and thirty compounds were identified using UPLC-ESI-QTOF-MS/MS, providing a better understanding of the polypharmacy of C. pareira.
- The study revealed that alkaloids may be responsible for the antiplasmodial activity of C. pareira root, opening up avenues for the development of novel drugs against malaria.
Chemical profiling and quantification of potential active constituents responsible for the antiplasmodial activity of Cissampelos pareira” is a research study conducted by CSIR-IHBT researchers Bhatt, Kumari, Upadhyay, Agrawal, Anmol, Sahal, and Sharma in 2020. The study focused on the evaluation of the anti-plasmodial potential of different crude extracts and fractions of C. pareira and the development of a UPLC-DAD method for the quantification of bioactive molecules. The researchers also identified isoquinoline alkaloids as potential active constituents responsible for the antiplasmodial activity of C. pareira root, providing a scientific basis for the traditional use of this plant for the treatment of malaria and opening avenues for future development of novel drugs.
Cissampelos pareira, a plant traditionally used in India as an anti-snake venom, may also possess anti-malaria properties. A recent study by Bhatt et al., published in the Journal of Ethnopharmacology, focused on evaluating the anti-plasmodial potential of different crude extracts and fractions of the plant, as well as developing a method for the quantification and identification of active compounds.
The study identified five compounds using UPLC-DAD and thirty compounds using UPLC-ESI-QTOF-MS/MS, providing a better understanding of the polypharmacy of C. pareira. The alkaloids found in the plant were revealed to be responsible for its antiplasmodial activity, opening up avenues for the development of novel drugs against malaria.
The study’s outcomes are significant because malaria continues to be a major public health challenge, with an estimated 229 million cases and 409,000 deaths globally in 2019. The potential anti-malaria properties of C. pareira represent a promising avenue for the development of new drugs against the disease. The study provides the scientific basis for the traditional use of this plant for the treatment of malaria, and opens up avenues for further detailed studies towards the development of novel drugs against malaria.
Chemical profiling and quantification of potential active constituents responsible for the antiplasmodial activity of Cissampelos pareira
Bhatt; Kumari; Upadhyay; Agrawal; Anmol; Sahal; Sharma 2020
Full-text link: https://doi.org/10.1016/j.jep.2020.113185
What this paper is about
- The leaves and roots decoctions as well as infusions are also used traditionally as anti-snake venom in India.
- Recently, reported the isolation, characterization and chemical profiling of isoquinoline alkaloids from the root of C. pareira.
- With the aim to investigate anti-plasmodial compounds of C. pareira, the present study was focused on evaluation of anti-plasmodial potential of different crude extracts and fractions, activity guided chromatographic isolation of alkaloids from C. pareira and, development of a UPLC-DAD method for the quantification of five and identification of thirty bioactive molecules in different parts of this plant.
What you can learn
- To better understand the polypharmacy of C. pareira, UPLC-DAD and UPLC-ESI-QTOF-MS/MS based quantification and identification of ingredients in the different parts and fractions of C. pareira was carried out and compared with the activity data.
- In this study, five compounds were quantified using UPLC-DAD and thirty compounds were identified using UPLC-ESI-QTOF-MS/MS.
- In conclusion, the present study revealed that alkaloids may be responsible for antiplasmodial activity of C. pareira root.
What is the traditional use of Cissampelos pareira in India?
Answer: The leaves and roots decoctions as well as infusions are traditionally used as anti-snake venom in India.
What was the aim of the present study on Cissampelos pareira?
Answer: The present study aimed to investigate the anti-plasmodial potential of different crude extracts and fractions, activity guided chromatographic isolation of alkaloids from C. pareira, and development of a UPLC-DAD method for the quantification of five and identification of thirty bioactive molecules in different parts of this plant.
How many compounds were identified in the study using UPLC-ESI-QTOF-MS/MS?
Answer: Thirty bioactive molecules were identified using UPLC-ESI-QTOF-MS/MS.
What was the conclusion of the study?
Answer: The present study revealed that alkaloids may be responsible for antiplasmodial activity of C. pareira root, and the promising activity of extract, fractions and pure isolated compounds against Pf3D7 and PfINDO strains of Plasmodium falciparum provides the scientific basis for the traditional use of this plant for the treatment of malaria and opens further avenues for the future detailed studies towards development of novel drugs against malaria.
Basics Q&A related to this research
Q: What is chemical profiling?
A: Chemical profiling is the process of identifying and characterizing the chemical components of a substance or sample.
Q: What is quantification in the context of this paper?
A: Quantification is the process of measuring the amount or concentration of a particular substance or compound in a sample.
Q: What are active constituents?
A: Active constituents are the chemical compounds present in a substance or sample that are responsible for its biological activity or therapeutic effects.
Q: What is antiplasmodial activity?
A: Antiplasmodial activity refers to the ability of a substance or compound to inhibit the growth or replication of Plasmodium parasites, which are responsible for causing malaria.
Q: What is Cissampelos pareira?
A: Cissampelos pareira is a plant species that is traditionally used in India for various medicinal purposes, including the treatment of malaria and as an anti-snake venom.
Q: What is isolation and characterization in the context of this paper?
A: Isolation and characterization refer to the process of separating and identifying specific chemical compounds or molecules from a complex mixture or sample.
Q: What are isoquinoline alkaloids?
A: Isoquinoline alkaloids are a class of naturally occurring chemical compounds that have a wide range of biological activities, including antiplasmodial activity.
